1) Soil sample were collected at depth of 20 cm with 5 times from each sub treatment. Soil
preparation was air dried and grind sieved at 2 mm.
2) Rhizomes were collected 7 times since August-February. Rhizome samples were kept in paper bag
before transport to laboratory and were rinse with clean water and dried in oven at 65-70 °C until its
completely dry. Then grinded with Wiley Cutting Mill and sieved though 40 mesh