Inoculum preparation for bacteria
Nutrient broth was prepared and sterilized in an autoclave at 15lbs pressure for 15 minutes. All the eight bacterial strains were individually inoculated in the sterilized nutrient broth and incubated at 370C for 24 hour. Nutrient agar (Himedia) was prepared, sterilized and poured into sterile petridishes and were left at room temperature for solidification. The 24 hour old bacterial broth cultures were inoculated in the petridishes using a sterile cotton swab.
Inoculum preparation for fungi
Sabouraud Agar (Himedia) broth was prepared and sterilized in an autoclave at 15lbs pressure for 15 minutes. The sterilized sabouraud agar was poured into sterile petridishes and incubated at 370C for three days. The fungal strain Candida albicans was inoculated in the broth using a sterile cotton swab and incubated at 370C for 72 hours