chromatographed over a
Sephadex LH-20 column (2.2 i.d. × 32 cm) with 70% EtOH(aq),
collecting 700-drop fractions, and yielded Sephadex fractions (Sfr) i-iii.
The Sfr-i (216 mg) was rechromatographed over the same Sephadex
column with 70% EtOH(aq) and afforded six subfractions (Sub) A-F.
The Sub B (18 mg) fraction was subjected to an MCI-gel CHP-20P
(1.1 i.d. × 21 cm) column with 25% MeOH(aq) and yielded pure