equipped with the vessels which are made of tetrafluoromethoxyl
and lined with Teflon liners. Rice powder (2.5 g)
was accurately weighed and placed into an extraction vessel.
According to the experimental design, a set volume and type of
solvent was added to the extraction vessel and the extraction was
performed under different MAE conditions. After extraction, the
vessels were allowed to cool down in an ice bath for 10 min and
carefully opened in a fume cupboard. Solid material from the sample
was separated from liquid, using a paper filter. The solid material
was thenwashed using new solvent. The liquid must be dried under
vacuum condition by a rotary evaporator, then re-dissolved with
methanol into 2 mL and filtered using 0.45 mm cellulose filter
(Millipore) prior to injection on the HPLCeFD system.