The applications of OS and EE synergistically delayed microbial growth. The preliminary test of showed that sponge cake packaged in Knylon bag with only EE showed visible mold growth after less than 7 day of storage. Therefore, the low concentration of ethanol, less than 0.2%, does not provide microcidal effect. On the other hand, the application of EE with OS (i.e., OS400 þ EE/K and OS400 þ EE/N) significantly extended the lag phase of aerobic microorganisms comparing to OS400K and OS400N with the same capacity of OS and packaging material.