However, there are some obvious drawbacks of GC methods. For example, long chain polyunsaturated fatty acids are unstable during the GC analysis, therefore, the accuracy was greatly affected [18]. It has been proposed that the injection technique, especially in vaporizing injectors, is the main source of error in quantitative GC [26]. Besides, the often used derivatizing reagent, such as boron trifluoride and diazomethane, are very dangerous or harmful. Compared with GC methods, the analysis of fatty acids by HPLC can be carried out at much lower temperature, therefore, the risk of damaging heat-liable compounds is greatly reduced. HPLC analysis also offers an alternative to GC for accurate quantitative analysis of fatty acids where GC is not available.