hese individuals were clearly
different fromC. opisthorchisin shape and noticeably larger than
C. orientalis. One specimen was from a frozenfish sample and not intact,
thus it was fixed in 70% ethanol and used for molecular analysis.
Another specimen was from a freshfish and still alive at the time of
collection, thus we made a whole mount specimen stained with
Heidenhain's iron hematoxylin as described above for morphological
observation and deposited at Meguro Parasitological Museum, Tokyo,
MPM Collection No. 21011