which was applied to each membrane in 1 g/L TBSV using TLC conjugate sampler (Sambrook
et al., 1989). The distance between the test line and control line was 6 mm. The test strips were dried at 37oC for 30 min. TBSV IgG–ovalbumin conjugate was diluted in 0.02 M sodium phosphate buffer (pH8.0) containing 1% sucrose to a final concentration of 3 μg/mL and applied as a 1mm thick, 26 cm line, at 0.5 cm from the top end of the membrane. The membrane was dried at 50oC under a dry