25OC and 60% relative humidity. Between 8 and 10 larvae were transferred to each cup,
the floor of which had been scratched into grooves and onto which the powdered artificial
diet was sprinkled. Crumpled tissue papers provided hiding places and moulting sites
and helped to reduce cannibalism. The Dixie@ cups were cleaned out at least once a week,
the old diet powder being removed and replaced with fresh, and the tissues and water
wick being removed, and replaced with clean ones. Larvae were allowed to pupate in
the Dixie@ cups and any remaining diet powder was carefully brushed out to avoid mould
contamination.