During the 1995 wet season, harvested rice seed was collected from
farmers’ fields at different locations in Iloilo, Philippines. Bacterial isolations
from crushed seed yielded 428 isolates. The isolates were characterized
by BOX-polymerase chain reaction fingerprinting of total
genomic DNA and represented 151 fingerprint types (FPT). Most FPTs
were found on a single occasion, although matching fingerprints for isolates
from different samples also were found. Identifications were made
by cellular fatty acid methyl ester analysis and additional use of Biolog
GN/GP MicroPlates and API 20E/50CHE systems. The predominant
bacteria were Enterobacteriaceae (25%), Bacillus spp. (22%), and Pseudomonas
spp. (14%). Other bacteria regularly present were identified as
Xanthomonas spp., Cellulomonas flavigena, and Clavibacter michiganense.
Of the total number of isolated bacteria, 4% exhibited in vitro
antifungal activity against Rhizoctonia solani or Pyricularia grisea. Two
percent of isolates were pathogens identified as Burkholderia glumae and
Burkholderia gladioli. Five percent of isolates induced sheath necrosis on
only 50 to 90% of inoculated plants and were related to Bacillus pumilus,
Paenibacillus spp., Pseudomonas spp., and Pantoea spp
During the 1995 wet season, harvested rice seed was collected fromfarmers’ fields at different locations in Iloilo, Philippines. Bacterial isolationsfrom crushed seed yielded 428 isolates. The isolates were characterizedby BOX-polymerase chain reaction fingerprinting of totalgenomic DNA and represented 151 fingerprint types (FPT). Most FPTswere found on a single occasion, although matching fingerprints for isolatesfrom different samples also were found. Identifications were madeby cellular fatty acid methyl ester analysis and additional use of BiologGN/GP MicroPlates and API 20E/50CHE systems. The predominantbacteria were Enterobacteriaceae (25%), Bacillus spp. (22%), and Pseudomonasspp. (14%). Other bacteria regularly present were identified asXanthomonas spp., Cellulomonas flavigena, and Clavibacter michiganense.Of the total number of isolated bacteria, 4% exhibited in vitroantifungal activity against Rhizoctonia solani or Pyricularia grisea. Twopercent of isolates were pathogens identified as Burkholderia glumae andBurkholderia gladioli. Five percent of isolates induced sheath necrosis ononly 50 to 90% of inoculated plants and were related to Bacillus pumilus,Paenibacillus spp., Pseudomonas spp., and Pantoea spp
การแปล กรุณารอสักครู่..
