We programmed incident light to cha

We programmed incident light to change as a sinusoidal wave over the course of the day. Light also varied from the surface of the illuminated culture to the bottom (Fig. 1).

The cells experienced a complex light environment due to the day/night cycle and also due to rapid vertical mixing from the constant stirring and sparging [25]. Our experiments started with approximately 2 × 106 Phaeodactylum cells mL− 1, a denser population than in natural habitats even during diatom blooms [43]. This led to self-shading effects and attenuation of light intensity through the 23 cm depth of the culture (Fig. 1, Fig. S1). Light attenuation with depth is also observed in large-scale photobioreactors or open ponds [44]. Additionally, the accumulation of biomass and cell pigments throughout the day increased the attenuation of light in the photobioreactor leading to a slightly asymmetrical pattern of light at the base of the culture (Fig. 1). Therefore our conditions mimicked light environments like those experienced by cells in a low density, well-mixed large-scale photobioreactor where cells are mixed turbulently through a broad gradient of light. Our conditions did not mimic conditions found in very dense cultures where cells will move rapidly between light and dark environments.

ผลลัพธ์ (ไทย) 1:

We programmed the incident light wave is a sine change the time of day the light is different from the culture surface brightness below (Figure 1) ambient light cells experienced the complex. Of the night, and because of continued rapid vertical mixing, stirring and sparging [25] Our experiments began with about 2 × 106 mL- cell population density of more than one data source in a natural habitat, even in the period. diatom blooms [43] This leads to self-shading and attenuation of the light intensity over the depth of 23 cm of culture (Figure 1, Figure S1) attenuates the light. The depth is observed. open ponds or photobioreactors large [44] In addition. The accumulation of biomass and pigment cells throughout the day increases the attenuation of light. photobioreactor That led to a slight asymmetry of the light at the base of the culture (Figure 1) , so mimic the conditions of our environment, such as the light of experience. The cells in the low density photobioreactor

ผลลัพธ์ (ไทย) 2:

We programmed the incident light to a sine wave at the time of the fire, is also different from the culture of the illuminated surface to the bottom (Figure 1) .. cells experience an environment with light. complicated because the day / night and also due to the rapid vertical mixing of continuous stirring and sparging [25] our experiments began with about 2 × 106 cells Phaeodactylum mL- 1 than in the population density. natural habitat even during diatom blooms. [43] This leads to the self-shading and reduce the intensity of light through a deep 23 cm of culture (Fig. 1 Fig. S1) light attenuation depth is also observed. photobioreactors Large open ponds or [44] In addition, the accumulation of biomass and pigment cells throughout the day, increasing the light attenuation in algae, leading to a form that can not balance a little bit at the base of the culture (Figure 1). So conditions. our imitation light environments such as those experienced by a cell in a large mixing low density. photobioreactor

ผลลัพธ์ (ไทย) 3:

We program the light to change is a sine wave over the course of the day. Light is also different from the surface to the bottom of the culture (Figure 1).Cells are complex lighting environment. The day / night cycle and due to rapid vertical mixing of constant stirring and sparging [25] Our experiments began with about 2 × 106 phaeodactylum cells ml - 1, with a population over a resident natural diatoms. blooms [43] this leads to a shading and their effects of light intensity over the 23 cm depth of culture (Figure 1, Figure S1) light attenuation with depth are also found in large ponds or photobioreactors. open [44] in addition, biomass accumulation and cell color throughout the day, making the attenuation of light. photobioreactor Leading to a slight asymmetry of the light at the base of the culture (Figure 1), so mimicked environmental conditions such as light, those with a low density of cells in the mixture. photobioreactor Large cell mixture through a severe slope width of the light. Our conditions do not mimic the conditions found in the cell culture density to move quickly between light and dark environments.