The total lipid extraction from the freeze-dried Chlorella sp. KR-1 was carried out using dimethyl carbonate and methanol
(7:3, v/v) under magnetic stirring at 60 °C for 12 h. After lipid extraction, the extraction mixture was filtered
to separate the lipid solution and lipid-extracted residual biomass.The residual biomass was dried at room temperature for three days.