Caucasian virgin dark brown hair tresses of 20.0 cm in
length, purchased from Bella Hair® (Brazil), were used.
Each hair strand was washed for 30 s with 15.0% (w/v)
sodium lauryl sulphate to remove impurities. All were
wetted with warm distilled water (37.0°C ± 2.0°C) constant
flow of 240.0 mL·min−1 for 1 min and the excess of
water was first removed by passing the tresses three
times between the fingers and then they were dried on
paper towel, for 12 h dried at room temperature (22.0°C
± 1.0°C) and relative humidity (RH 60% ± 5%) prior to
the analysis [12]