2 and 3 show the results obtained after assessing the effects of different bacterial loads on total and progressive sperm motility, respectively. P. aeruginosa affected sperm motility in a both time- and concentration-dependent manner. There was also an interaction (P < 0.05)between these two factors (i.e. bacterial load × storage time).were found (debris). Sperm viability was calculated as the mean of three measures performed per sample (mean ± S.E.M.).