The study revealed that SL as a learning environment can be utilized as a tool for spatial learning in interior architecture and can aid interior architects in the design process. In SL, participants were able to navigate from an egocentric viewpoint and learn the spatial environment. They indicated that they had a sense of presence in the VE and their virtual navigation behaviors were similar to the real world environment. It can be stated that spatial knowledge acquired from the VEs can be effectively transferred to real world environments. During the design process, interior architects need to consider the navigation problems of the individuals; SL can be utilized as a tool for understanding the spatial environment and allowing interior architects to obtain an immersed view of the proposed building, as well as assess and improve their designs. In addition, SL enables interior architects to learn from the navigation behaviors of male and female users in order to overcome potential navigation problems that individuals will encounter in a spatial environment. Further research can be conducted with design students to understand the effectiveness of SL as a design environment in interior architecture design education.