Five grams of extract was mixed with 200 mL of 10% acetic
acid in ethanol and allowed to stand for 4 h. Mixture was sieved
and the extract was concentrated on a water bath to one quarter
of the original volume. Concentrated ammonium hydroxide was
introduced drop wise to extract until precipitate was complete.
The whole solution was settled and the precipitate was washed
with dilute ammonium hydroxide and then filtered. The residue
which was the alkaloid was desiccated a