The automatic TLC sampler 4 (ATS 4) from Camag, Fig. 4, is an example of a fully programmable automated sample applicator that can apply samples as spots (0.1–5 L) by contact transfer or as bands or rectangles by the spray-on technique (samples >0.5 L). Samples are housed in a vial rack (66 sample vials) or 96-well plate and can be selected in any order for application to the layer. Samples scan also be applied in sequence as an overlay of sample and standard (standard additions). The syringe is automatically emptied and rinsed between sample applications. A heated spray nozzle (at 60◦C) is available to facilitate the application of aqueous samples (and similar moderate volatility solvents). A recent upgrade (ATS4 FREE MODE) was introduced for the application of reagents to diagnostic strips with different shapes and for the programmed application of samples to multiple plates.