This study was conducted to antagonistic formulation of Bacillus subtilis, Bacillus amyloliquefaciens biomass with powder, NB and sticking agent Magnesium sulfate, Carboxymethyl cellulose, and Talcum were conducted to test for viable cell. The results showed that, the formulation contain number of viable cells at 10-8 cfu/ml at 2 month. For the application of the formulation for control anthracnose causing Collectotrichum sp. with spraying pathogenic fungi after antagonistic bacterium, sticking agent formulation was more effective to control the disease than control on peppers seed.