1. Two glass pages were set in a glass caster for preparing gel.
2. The 15% of separating gel was loaded into the glass pages and wait for 20 minutes.
3. The stacking gel was loaded and put the comb at the top of the cast gel, waits for 20 min.
4. Remove the comb from the cast gel.
5. Wash the gel with DI-water to remove the excess acrylamide solution.
6. Transfer the glass plate set into the module.