Lignocellulose-derived microbial lipids are considered as a potential alternative to vegetable
oil as a solution to the feedstock shortage problem that prevents the large-scale production of
biodiesel [1]. Three major processes are generally required in order to make lipids from
lignocellulosic biomass, namely pretreatment, hydrolysis and lipid production [2,3]. The
hydrolysis process uses various enzymes to produce synergistic effects [4]. Although the cost
of enzymes has been continuously dropping due to improved specific enzymatic activities,
enhanced stability and increased production efficiency, it remains a major obstacle to
reaching that required for viable commercial production of biofuels and commodity
chemicals from lignocellulosic biomass.