Amino acids in BPC and a selected BPH (20%
DH) were determined. The hydrolysate (40 mg)
was dissolved in 6 N HCl (15 ml). Then 100 µl
sample solutions were taken and derivatived using
EZ:Faast™-Amino acid analysis Kit. The amino acids
were separated by HPLC-MS. (1100 SL, Agilent,
CA, USA) at 35o
C, using gradient mobile phase:
eluent A (10 mM Ammonium formate in water) and
eluent B (10 mM Ammonium formate in methanol).
The amounts of amino acids were calculated, based
on the peak areas in comparison with that of the
standard. Each amino acid content was expressed as
a percentage of total amino acids in the sample.