Compounds having a high diffusion coefficient or solubility and low antimicrobial activity may penetrate the agar rapidly,even in small amounts, and give zones like those of active compounds with poor penetration. This problem is encountered when zones of inhibition are compared for different classes of compounds. Additionally, size of inhibition zones might be influenced by volatilization of antimicrobial substances, disk size, amount of compound added to disk, adsorption by the disk, type of agar, agarstrength, pH, volume of agar, and microbial strains used(Pauli, 2006). In our study, this method was not sufficiently