Preparation of the Extract
Two different fresh onion extracts were made
from onion bulbs using sterilized distilled water. The
extracts were cold water extract and fresh onion
extracts.
For the preparation of cold water extract, 5
onion bulbs were peeled, rinsed with distilled water and
cut into small pieces. The moisture of the onion was
removed by drying inside oven at constant temperature
of 600C. The resulted onion crimps was grinded using
mortar and pestle. 11.9g of the onion powder was
weighed and was dissolved into a conical flask
containing 100mls of distilled water. The mixture was
sterilized using autoclave at 1210C for 15 minutes and
was left to soak for 2 days after which it was filter
aseptically and was transferred in to sterile bottle.
The fresh onion extract was prepared by
blending five bulbs of onion that was surface sterilized
by using 70% alcohol and rinsed-off with sterile
distilled water. The liquid content was sieved
aseptically and collected with sterile bottle. All extracts
were properly stored at 40C throughout the
experimental period.