2. Materials and methods
2.1. Reagents
Glucose oxidase (EC 1.1.3.4, Type VII from Aspergillus
niger) was obtained from Sigma (MD, USA). Ultra-thin dialysis
membrane (Part No. 157-0144-02; thickness; 15 m) was
purchased from Technicon Chemical Co. (Orcq, Belgium).
Photosensitive polymer (azide-unit pendant water-soluble photopolymer
(AWP)) was obtained from Toyo Gosei Kogyo Co.
(Tokyo, Japan). All other reagents used for experiments were
commercial- or laboratory-grade. Glucose was dissolved in0.1M phosphate-buffered solution (PBS; pH 7.0), titrated and
stored for ≥12 h to prepare standard glucose solutions.