The CEY concentration 4% (INRA4) leads to decline of motility and membrane and functional integrity. Additionally, the defined soybean lecithin-based extender with centrifuged CEY 2% facilitates motility evaluating in CASA because it has less viscous fluid. Furthermore, the results show that freezability of Caspian horse semen can be affected by EY concentration and type (centrifuged versus whole).Finally, the present results suggest that the use of a low concentration of CEY (2%) and soybean lecithin in combination significantly improved reproductive traits of Caspian horse semen, indicating its beneficial effect during the freeze-thaw process. Nevertheless, further studies should be carried out in order to confirm with AI trials of this presented results.