In this study, an amperometric biosensor was developed in order
to investigate the effect of calcium on the activity of catalase
enzyme. From the experimental studies we detected a positive effect
of calcium on the enzyme activity and this effect increased in
higher calcium concentrations. By using the biosensor we detected
a linear concentration range for calcium in the presence of constant
concentration of hydrogen peroxide. The biosensor is really original
for Ca determination especially liquid samples and there is
no any study like this in the literature. It does not need any expensive
equipments, materials or laboratory conditions. The biosensor
is portable so it can be used in everywhere for Ca analysis. For all
liquid samples it is not necessary any pre-treatment for the samples
except dilution (if it is necessary) in the Ca determination.
The biosensor developed can be used not only food samples also
clinical purpose. In dealing with a large number of samples, the
biosensor is rapid, accurate, precise and with low operation cost
is required. By using the biosensor because of the specificity of
the enzyme we can determine calcium concentration in the presence
of the other metal ions such as Cu2+, Mg2+, Ni2+, Na+
, Mn2+
and K+
. Reproducibility of the biosensor is very well and from the
application studies of real samples it can be said that the biosensor
can be used as a sensitive alternative method for analysis of
calcium.