E. hirta extract effectively eradicated the established biofilm of P. aeruginosa, with minimum biofilm eradication concentration (MBEC) value of 0.5 mg/ml. The methanol extract also showed weak anti-biofilm activities against E. faecalis and P. aeruginosa (ATCC 27853) exhibiting MBEC value of 1.0 mg/ml. Nevertheless, E. hirta extract displayed no capability to disrupt the established biofilm in the rest of the selected pathogens at the highest test concentration of 1 mg/ml. Cefepime found to be ineffective to eradicate biofilm of all the tested pathogens except for the clinical isolate, P. aeruginosa with MBEC value of 0.5 mg/ml.