Optimisation of different culture conditions
for the enhanced cell growth and CPT production
in OMC3 cell line
Inoculum size
Inoculum size of the culture system against fixed volume of
nutrient media (50 mL) was studied. Varied inoculum sizes of
cells in 50 mL nutrient media were tested, and the results obtained
are given in Table 1. It was found that 14 g L−1 (0.7 g/flask)
was required for obtaining maximum biomass (10.5 ± 0.8 g/flask
FW) and CPT production (0.143 ± 0.005 mg g−1 DW) over 25 days of incubation. When inoculum size was small, cell
growth was very low. It was also noted that the biomass yield
had significantly decreased when the inoculum size was above
14 g L−1. CPT production was remarkably affected by both low
and high inoculum size.