Dried
peel was then taken for grinding by blender and powdered
form of plant sample was soaked in 95% ethanol, 40%
ethanol (rice whisky)or acetone in a ratio of 1:8 in the
dark, at room temperature for 3 days separately. The mixtures
were filtered through a sterile filter cloth and a sterile filter
paper (Whatman No. 1), respectively. The filtrates
obtained were subsequently concentrated to a small
volume under vacuum on a rotary evaporator (Laborata
4000; Heidolph Instruments GmbH & Co. KG, Germany).