2.4.3. Inhibition of hemolysis in human erythrocytes
Human blood samples were obtained from the Biochemistry Department of
Félix Guyon Hospital (Saint Denis, Réunion, France). Using the modified method
of Prost (1992), erythrocytes were isolated, washed and diluted in 0.15 M NaCl.
To 100 ll (2 107 erythrocytes/ll) of diluted red blood cell solution, 100 ll serum
extract obtained from the clinical trial (serially diluted in 0.15 M NaCl) or FPP (200–
1000 lg/ml) was added. Lysis of erythrocytes was initiated by adding 40 ll 2,20-
azobis(2-methylpropionamidine) dihydrochloride (AAPH, 0.5 M). Turbidity at
450 nm was recorded at intervals of 10 min using a thermostated microplate
reader.