The mixture was stirred thoroughly, and then subjected to microwave
treatment (300 W) at 200 C for 5 min (Tatijarern et al.,
2013). The mixture was left to cool at room temperature. The pH
of the hydrolyzate was adjusted to 4.8 using 40% w/v NaOH. Cellulase
from T. reesei ATCC 26921 (160 lL/1 g of grass) was added into
the liquid hydrolyzate. The hydrolyzate was incubated for 60 h at
50 C (Qureshi et al., 2008).