The various extracts of leaves Holoptelea integrifolia (Ulmaceae) were investigated for analgesic activity in
mice by tail flick method. The fresh plant leaves of H. integrifolia were collected, dried, cleaned, weighed and chopped
into small pieces and percolated in ethanol. The fractionation of crude extract, followed by the addition of distilled water,
ethyl acetate and n-butanol to an aqueous portion of each solvent, to obtain the dried masses of each four layers.
Qualitative chemical examination indicates the presence of secondary metabolites such as alkaloids, flavones, phenol,
steroids, tannins and triterpenoids. No acute oral toxicity was observed and extracts considered being safe at the dose of
50-2000 mg/kg body weight. At the dose of 500 mg/kg various extracts of leaves of H. integrifolia were found
statistically significant (P<0.05). A maximum effect was established at 150 min, after drug administration. Diclofenace
sodium used as a standard.
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