Results from Pz-ph evaluation suggested that carrying out measurements in triplicate for phospholipase is not essential
to obtain adequate intra-examiner reproducibility.
Thus, it seems that accurate measurements were more easily obtained in the analysis of phospholipase, which
may be explained by the characteristics of the colony and halos formed. The methodology used promoted the formation
of large colonies and halos with well-defined borders, which did not occur in the SAPs analysis (Figure 3).
Moreover, the use of egg yolk with potassium tellurite allowed the formation of dark colonies contrasting with the
yellow color of the surrounding halos, which possibly improved the visualization of the halos of degradation and
the accuracy of measurements. In the literature, few studies stated to have measured only one colony.11,57 On the
other hand, there were more studies carried out in triplicate but there was no information
about the calibration of the researchers, which makes it dif-ficult to discuss the accuracy of the measurements taken in
each case.