Leaf explants were cultured in 20 different treatments of plant growth regulators (NAA, BA, TDZ and 2,4-D) in order to initiate callus formation. The result showed that Gamborg B-5 medium supplemented with 1 mg/L TDZ and 0.1 mg/L 2,4-D gave the highest callus formation (83%). We tried to regenerate the shoots from these callus by subculturing to Gamborg B-5 medium with different plant growth regulators but we found no shoot formation from these callus.