Biocontrol agents
Two isolates of yeasts P. albicans and C. sake were isolated from the
surface of orange fruits following protocol described by Fiss et al. (2003) for
the isolation of epiphytic fungi. The isolates were grown at 27 °C on a complex
medium (10 gm malt extract, 4 gm yeast extract, 4 gm glucose, and 15 gm agar
per liter) and transferred every 6 weeks. The first identification was based on
the key of Van der Walt and Yarrow (1984). Additional morphological,
physiological and biochemical characters of the isolated yeasts were obtained
by the methods currently used in yeast taxonomy (Yarrow, 1998). The yeast of
S. cerevisiae obtained from Arab company for medicines and medicinal plant
(Meba Co., Egypt). Rhizo-N (Bacillus subtilis) was provided from El-Nasr
Company for fertilizers and biocides in El-Sadat city, Egypt.