Since L. japonica hydrolysate (Fig. 1) contained a high amount of
mannitol, the ability of E. coliKO11 andS. cerevisiaeto ferment this
sugar was determined. The yeast was unable to ferment mannitol
whereasE. coliKO11 produced as much as 25.8 g ethanol /L after
116 h of cultivation when provided with 75 and 90 g/L of mannitol
(Fig. 2).E. coliKO11 could ferment mannitol to ethanol up to a yield
of 0.41 g ethanol/g mannitol. This yield was higher than that of the
yeastZymobacter palmae(0.38 g ethanol/g mannitol) (Horn et al.,2000a,b). Since 90 g/L of mannitol did not severely inhibit growth of ethanogenicE. coliKO11, higher ethanol yield from mannitol
can potentially be achieved if in situ ethanol is removed, for example by using a membrane system (Lee et al., 2008a).E. coliKO11 is also able to produce ethanol when 19.6 g/L of mannitol and
19.5 g/L of glucose were provided (Fig. 3), although the consumption rate of mannitol was lower than that of glucose (0.41 g mannitol/h vs. 0.65 g glucose/h)
Since L. japonica hydrolysate (Fig. 1) contained a high amount of
mannitol, the ability of E. coliKO11 andS. cerevisiaeto ferment this
sugar was determined. The yeast was unable to ferment mannitol
whereasE. coliKO11 produced as much as 25.8 g ethanol /L after
116 h of cultivation when provided with 75 and 90 g/L of mannitol
(Fig. 2).E. coliKO11 could ferment mannitol to ethanol up to a yield
of 0.41 g ethanol/g mannitol. This yield was higher than that of the
yeastZymobacter palmae(0.38 g ethanol/g mannitol) (Horn et al.,2000a,b). Since 90 g/L of mannitol did not severely inhibit growth of ethanogenicE. coliKO11, higher ethanol yield from mannitol
can potentially be achieved if in situ ethanol is removed, for example by using a membrane system (Lee et al., 2008a).E. coliKO11 is also able to produce ethanol when 19.6 g/L of mannitol and
19.5 g/L of glucose were provided (Fig. 3), although the consumption rate of mannitol was lower than that of glucose (0.41 g mannitol/h vs. 0.65 g glucose/h)
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Since L. japonica hydrolysate (Fig. 1) contained a high amount of
mannitol, the ability of E. coliKO11 andS. cerevisiaeto ferment this
sugar was determined. The yeast was unable to ferment mannitol
whereasE. coliKO11 produced as much as 25.8 g ethanol /L after
116 h of cultivation when provided with 75 and 90 g/L of mannitol
(Fig. 2).E. coliKO11 could ferment mannitol to ethanol up to a yield
of 0.41 g ethanol/g mannitol. This yield was higher than that of the
yeastZymobacter palmae(0.38 g ethanol/g mannitol) (Horn et al.,2000a,b). Since 90 g/L of mannitol did not severely inhibit growth of ethanogenicE. coliKO11, higher ethanol yield from mannitol
can potentially be achieved if in situ ethanol is removed, for example by using a membrane system (Lee et al., 2008a).E. coliKO11 is also able to produce ethanol when 19.6 g/L of mannitol and
19.5 g/L of glucose were provided (Fig. 3), although the consumption rate of mannitol was lower than that of glucose (0.41 g mannitol/h vs. 0.65 g glucose/h)
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