The plate was grown in a microaerobic
atmosphere (5% O2, 10% CO2, 85% N2) at 37 C for 5 days. The typestrain
of K. denitrificans ATCC33394 was obtained from the American
Type Culture Collection. K. denitrificans KDY1was isolated from
a nasopharyngeal swab of a leukemic patient and H. pylori CPY3401
was from gastric biopsy specimen [18] in Yamaguchi University
Hospital, respectively. And HPT73 is an isogenic ureB-disrupted
mutant of CPY3401. The culture condition of K. denitrificans was
exactly the same as that of H. pylori.