1. Most bacteria and fungi can be growii in a variety of artificial media,
but strictly intracellular microorganisms (eg, Chlamydia, Rickettsia, and viruses) can be isolated only in cultures of living eukaryotic cells.
2. The culture of some parasites is possible, but used only in highly
specialized laboratories.
“The success of culturing depends on appropriate collection and transport .
techniques, and on selection of appropriate culture media.