Incubation time
Incubation time also have a significant effect on the production of biosurfactant. Different microorganisms are able to produce biosurfactants at different time intervals. The maximum biosurfactant production by Aspergillus ustus MSF3 was observed after 5 days of incubation while in case of Candida bombicola, the incubation periods were 7, 8 and 11 days [7,13,12]. Also, the maximum biosurfactant production by Candida bombicola using animal fat was observed after the 68 h of incubation