The objectives of this study were to develop cost-effective and time-saving methods for screening of GMOs and to determine the suitability of these methods for food analysis.Here,we propose four new DNA markers targeting important GMO specific sequences,such as cauliflower mosaic virus (CaMV) 35 Spromoter,NOS terminator, epsps gene,and cry1Ab gene. In addition,new multiplex PCR methods allowing simultaneous detection of the herbicide tolerance GM trait (CP4-EPSPS for RoundupReady),CaMV 35 S promoter,NOS terminatorand soybean specific lectin gene as well as simultaneous detection of the insect control GM trait(Cry1Ab),CaMV35 S promoter
and maize specific zein gene are presented and discussed.The application of these methods to GM reference materials as well as different food products is described and explained.