Serum iron, total iron binding capacity (TIBC) and
transferrin saturation were estimated using a
ferrozine–based iron/TIBC reagent set (Pointe
Scientific, USA). Test procedures were conducted as
described in the manufacturer's standard operating
manual included with the kit. Transferrin saturation
was calculated from the serum iron concentration and
TIBC values as follows: transferrin saturation = serum
iron/TIBC x 100