The present study adopted a proteomics approach to discriminate between the expression of different
proteins in meat exudates from fresh and freeze-thawed (FT) pork. The porcine longissimus thoracis
muscles were stored at 1.0 ± 0.5 C for 60 h, and the remainder was exposed to freeze-thawing
at 20.0 ± 0.5 C for 48 h and at 1.0 ± 0.5 C for 12 h. The released meat exudates were collected,
and the amount of exudate and the protein content and expression were analyzed. Two-dimensional gel
electrophoresis and mass spectrometry (2DE/MS) identified 450 protein spots from meat exudates. The
expressions of 15 and 9 proteins were significantly (p < 0.05) higher in the fresh pork and in FT pork,
respectively. 22 proteins could be selected as markers to discriminate between fresh and FT pork