In addition to these substrates, a common laboratory culture medium (TSB) was also used to assess potential differences in adherence/attachment and/or biofilm formation as compared to real food substrates. Temperatures, representative of beef fabrication areas during non-production hours (15 c) and cold meat storage (14 c), were also incorporated in this study to investigate the adherent properties of E. coli O157:H7 in cold and moderately cold environments.