The model equations predicted by RSM indicated that both
reaction temperature and flow rate were significant factors for
xylan recovery, while only reaction temperature was significant
factor for xylan purity in the second stage. The predicted
value of xylose recovery yield using RSM was 52%, and the
value of purity was 82% under the optimal reaction conditions
respectively. From the statistical results, the model equations
for xylose recovery yield and purity were significant. Experimental
verification of the optimal reaction conditions showed
similar values of xylose recovery yield and purity: 55% and
84% respectively.
Cellulase enzymes hydrolyzed two-stage treated solids
into fermentable sugars effectively. Accordingly, high yields of
ethanol fermentation were obtained using stable S. cerevisiae
yeast (D5A). Xylooligomer hydrolyzate obtained from twostage
fractionation was effectively hydrolyzed by xylanase
enzyme.
This study demonstrates optimization of a lignocellulosic
biomass processing method that allows the production
of ethanol from cellulose and the production of other
value-added co-products from hemicelluloses sugars and
lignin.