2.3. Experimental procedure in primary cultures of rat
hepatocyte
Primary cultures of rat hepatocyte were prepared as previously described by Pramyothin et al. (2005). After 24 h of
culturing, hepatocytes were treated with ethanol (96l/ml) and
PA (1–4 mg/ml) for 2 h. After 2 h incubation, cell viability was
tested using MTT assay, AST and ALT release.
All experiments were performed at least three times using
different cell preparations.