1 - organizes and defines the field microscopy exactly and then transmits the amount of 0.01 ml of milk or food liquid to a glass slide especially clean and publish an area of cm 2 (100 mm 2) and then let the liquid that dries out and prove the membrane by heat and dying by methylene blue and then counting according to the number of bacterial or Microbial clump per field after counting 10-60 field.