The present investigation brings forward the exciting revelations of varied in vitro cultural behaviour of seed explant
in terms of seed germination, callus, shoot, and root development in response to different culture conditions. In ten
different hormonal combinations tested, the best germination
response was observed in Mitra medium supplemented with
2.0 mg/L BAP and 2 mg/L IAA. While highest leaf formation
for 13 weeks old culture was recorded in medium enriched
with 2.0 mg/L KN and 0.5 mg/L NAA, the maximum rooting
response was induced in 3.0 mg/L KN and 1.5 mg/L NAA
integrated medium. The enrichment of medium with higher
concentration of IBA or NAA along with low cytokinins
content induced excellent rooting response in the culture.
BAP and KN on the other hand produced more pronounced
shooting multiplication and leaf formation when associated
with lower concentration of auxins rather than existing singly.
The in vitro seed culture protocol established from the present
study can be employed for large scale propagation of this
medicinally important multiutility Dendrobium orchid for
commercial and conservation purposes.