Fossil fuels are being used at an alarming rate and substantial
amount of waste is being generated worldwide. Waste/Wastewaters
are being considered as a replacement to non-renewable resources,
especially for the generation of wealth in the form of biobased
materials, fuels and energy. Therefore, in the present work wastewater
was used as a substrate for PHA production. The influence of
reactor microenvironment and operating pH on PHA production
was evaluated. Functional role of dehydrogenase enzyme activity,
electron discharge properties and bio-electro kinetic analysis of
mixed culture on PHA producti on was also evaluated. Neutral pH
operation at microaerophilic environment showed higher PHA
accumulation than other conditions. The co-polymer P(3HB-co-
3HV) obtained showed higher hydroxybutyrate content. Bioprocess
monitoring and wastewater treatment showed good correlation
with the PHA production. These findings depict an economically and
environmentally attractive method for optimizing mixed culture
PHA production using wastewaters and provide impetus for further
process advancements.