1. Introduction
The potato (Solanum tuberosum L.) is an economically
important plant used as food in many countries of the world
and also very important plant for the Turkish economy grown
over a large area. The potato is the first major food crop in
which biotechnology has been successfully implemented for
seed production. Previous studies show that micropropaga-
tion of potatoes depends on the biological value of cultivars,
explant type (leaf, node, shoot tip, etc.), type of culture
medium, season, temperature, photoperiod, and a balanced
combination of plant growth regulators (PGRs) in the culture
media [1–3]. Axillary buds, nodal tissues, stem explants,
roots, leaves, tubers, shoots or stems, and cell suspension
cultures have been utilized to micropropagate potatoes [1, 4].
The successful in vitro multiplication of potatoes depends
on the presence of a suitable combination of auxins with
gibberellic acid (GA3 ) in the propagation medium [4–7].
Roest and Bokelmann [8] suggested that a lower concen-
tration of auxin with GA3 (0.25 mg L−1 ) had a positive
impact on the shoot and root development of potato plantlets
grown in vitro. Ghaffoor et al. [9] have also suggested that
the longest shoots were noted on