Cycloheximide
at a concentration of 0.01% (v/v) was added into enumeration
agar plate to prevent fungal growth. After incubation, plates with
30–300 colonies were enumerated and recorded as colony forming
units (cfu/mL) of samples. MRS and M17 plates were incubated
anaerobically at 30 ◦C using anaerobic jars together with the BBL
(Baltimore Biological Laboratory, GasPak 100 Anaerobic system, BD
Bioscience) GasPak EZ Anaerobic Pouch. Colonies with distinct morphologies
differences (such as color, shape and size) were selected
randomly and purified by streaking using the same medium. All
isolates were checked for the catalase reaction and examined
microscopically before stock preparations. Gram-positive, catalasenegative
bacterial isolates were purified and frozen stocks were